The di Rosa Preserve presents a rare opportunity to acquire one of Napa Valley's most iconic and irreplaceable cultural estates. Comprising approximately 217 acres across two legal parcels, the property blends expansive open space, vineyard and lake views, historic structures, and highly valuable legacy Napa County Use Permit entitlements. The Preserve includes the 52.5 acre Winery Lake parcel, featuring a 32 acre reservoir (320 acre-feet capacity), cultural and historic buildings, galleries, and event infrastructure, along with the 163.5 acre Milliken Peak parcel, offering dramatic elevation, panoramic views, open space, and outdoor art and hiking experiences. Conservation easements held by the Napa Valley Land Trust protect the property's scenic and cultural character while allowing continued permitted uses. A rare and transferable Napa County Use Permit allows for public visitation, cultural exhibitions, retail, and up to 36 events annually (up to 250 guests per event), with the ability to pursue larger gatherings via Temporary Use Permits, subject to County approval. Existing operations have included tours, exhibitions, workshops, weddings, and fundraising events. The property is ideally located directly across from Domaine Carneros along one of Napa Valley's most traveled and prestigious corridors, offering exceptional visibility and accessibility just minutes from Downtown Napa and Sonoma Plaza. Additional features include vineyard lease income, shared access and water agreements, established parking and traffic approvals, wildlife sanctuary designation surrounding Winery Lake, and grazing and open-space uses on Milliken Peak. Future uses are subject to existing conservation easements, use permit conditions, and Napa County review. A truly once-in-a-generation offering combining land, legacy entitlements, cultural significance, and irreplaceable location in the heart of Napa Valley.